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西南大學發表文章中用zeta life轉染試劑高效率轉染HT-29細胞
發布時間： 2021-07-23 點擊次數： 2414次西南大學用zeta life轉染試劑高效率轉染HCT116、SW480、HT29人結直腸癌細胞，其發表文章已見刊。
西南大學生命科學學院教育部創新重點實驗室
使用zeta life，Advanced DNA RNA轉染試劑
高效率轉染HCT-116, SW480, HT-29人結直腸癌細胞
發表文章轉染條件
A、HCT-116, SW480, HT-29人結直腸癌細胞
B、AURKA質粒
C、轉染細胞融合度50%
D、96孔板每孔使用0.5μg質粒DNA
6孔每孔使用10 ug質粒DNA
(注意：本實驗中用到的細胞密度、轉染質粒DNA用量不適用于LIPO3000/2000)
發表文章部分內容
Overexpression of AURKA Plasmid for AURKA overexpression was obtained from VectorBuilder. Details about plasmid containing the AURKA gene can be found at . colon cancer cells (HCT-116, SW480, HT-29) were seeded at 50% confluency for 24 h in six-well and 96-well plates. Plasmid (10 ug per-well for six-well and 0.5 μg per-well for 96 plates) and the Advanced DNA RNA transfection（zeta life,USA) reagent were mixed in equal proportionsand added to the cell culture medium for 6 h. DOX (1 μg/ml) was added to induce the expression of AURKA. After addition of DOX and PAL for 36 h, cell viability was measured by MTT and apoptosis was detected by。
PAL exerts anti-colon cancer effects by targeting AURKA. (A) PAL promotes apoptosis in colon cancer cells (HCT-116, SW480) in a dose-dependent manner. (B) PAL promotes G2/M phase arrest in colon cancer cells (HCT-116, SW480) in a dose-dependent manner. (C) The expression of AURKA after DOX treatment for 24 h. (D) Cell density after PAL treatment for 24 h in the cells (HCT-116, SW480) transfected with AURKA overexpression plasmids. DOX is an inducer of AURKA expression in plasmids. (E) Effect of PAL detected by MTT assay on the proliferation ability of colon cancer cells transfected with AURKA overexpression plasmids. (F) Effects of PAL detected by.
深圳市安培生物科技有限公司是美國ZETA LIFE的中國深圳地區總代，我司代理其Advanced系列高效DNA、RNA轉染試劑。在細胞轉染實驗中均有高效轉染率，我司目前現貨供應，助力每個科研人實現科研夢。公司的宗旨：致力成為推動生命科學進步的和值得信賴的合作者。
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